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rnascope probe m. musculus rspo2  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope probe m. musculus rspo2
    Rnascope Probe M. Musculus Rspo2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+probe+m%2E+musculus+rspo2/rnascope+probe+m++musculus+rspo3/10__7554_slash_elife__59142-167-72-78
    Average 90 stars, based on 1 article reviews
    rnascope probe m. musculus rspo2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Positive Control:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Negative Control:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Sequencing:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    RNAscope:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Expressing:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    RNA Sequencing:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Immunofluorescence:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Staining:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Marker:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Biomarker Discovery:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    In Situ:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Hybridization:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Isolation:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Recombinant:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .

    Software:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo2 , Advanced Cell Diagnostics , 402001 , .



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